1. Pest Manag Sci. 2016 Aug;72(8):1529-36. doi: 10.1002/ps.4181. Epub 2015 Dec 1.
Evaluation of the endophytic nature of Bacillus amyloliquefaciens strain GYL4 and
its efficacy in the control of anthracnose.
Kim JD(1), Jeon BJ(1), Han JW(1), Park MY(1), Kang SA(1), Kim BS(1).
Author information:
(1)Laboratory of Plant Pharmacology, Department of Biosystems and Biotechnology,
College of Life Sciences and Biotechnology, Korea University Graduate School,
Seoul, 136-713, Republic of Korea.
BACKGROUND: Endophytic bacteria are viewed as a potential new source of
biofungicides because they have beneficial characteristics as control agents for
plant disease. This study was performed to examine the endophytic feature and
disease control efficacy of Bacillus amyloliquefaciens strain GYL4 and to
identify the antifungal compounds produced by this strain.
RESULTS: B. amyloliquefaciens strain GYL4 was isolated from leaf tissue of pepper
plants (Capsicum annuum L.). Anthracnose symptoms were markedly reduced in the
leaves of pepper plants colonised by GYL4. An egfp-expressing strain of GYL4
(GYL4-egfp) was constructed and reintroduced into pepper plants, which confirmed
its ability to colonise the internal tissues of pepper plants. GYL4-egfp was
observed in the root and stem tissues 4 days after treatment and abundantly found
in the internal leaf tissue 9 days after treatment. Bacillomycin derivatives
purified from the culture extract of GYL4 displayed control efficacy on
anthracnose development in cucumber (Cucumis sativus L. cv. Chunsim).
CONCLUSION: The present study is the first report on evaluation of the endophytic
and systemic nature of B. amyloliquefaciens strain GYL4 and its potential as a
biocontrol agent for anthracnose management. © 2015 Society of Chemical Industry.
© 2015 Society of Chemical Industry.
DOI: 10.1002/ps.4181
PMID: 26518268 [Indexed for MEDLINE]