Once it shows its first set of true leaves, depending on your product, I use superthrive for root growth for example, I use a very very small amount, like .5 ml per gallon. So about a drop per 4 ounces or so.
It contains no fertilizer just vitamins and stuff.. very good for transplanting. But your roots will go nuts, I have plants that are the youngest in my grow that are completely rooted to the top of a 5 gallon pot.. makes it very hard to break up the top of the dirt before watering. I used it when I transplanted at a slightly stronger concentration. They rewarded me too much. Go easy and you will be fine.