When you see growth within hours of transplant, you know. It means the plants has sensed it has more room to send out root. If it was shocked it would and does, stop growing. I personally think that the worst way to transplants autos is the "tier" method. First, most cannabis only has a taproot a couple inches long, the rest is regular food and water gathering roots. Second, you want your roots to spread out, width matters more than depth. Forcing all the roots out through a 3" diameter opening is restrictive.
And maybe my documentation wouldn't measure up to scientific standards, but, I've done comparison experiments with seed and clone plants, transplanted, and started in the final pot, all at the same time. I've found that transplants will equal the one pot plants 85% of the time, 10% exceeding the one pot plant, and 5% with slightly less growth, usually only in plants that I damaged the rootball of in transplant. My observations were not casual, I spent months studying this.
And maybe my documentation wouldn't measure up to scientific standards, but, I've done comparison experiments with seed and clone plants, transplanted, and started in the final pot, all at the same time. I've found that transplants will equal the one pot plants 85% of the time, 10% exceeding the one pot plant, and 5% with slightly less growth, usually only in plants that I damaged the rootball of in transplant. My observations were not casual, I spent months studying this.
Transplanting may stunt your growth but how would you ever know? Just because they don't die does not mean it was fully successful. I simply do not have enough farm space to do a truly scientific cannabis test. Extrapolating my 40+ years experiences with growing organic vegetables and cutting flowers I can state that any kind of transplanting reduces yields compared to seeds planted directly into the garden. Seeds started in greenhouses to get a jump on spring just do not produce as well. I am sure there are other factors besides "transplanting" working here. The question is this: why can't you just plant the seed in the container it will spend its whole life in? I do. My current grow is an exception to that practice as I have never grown in rockwool blocks so I followed GroDan's directions to the letter. The transplanting process is very root friendly but still is a transplant operation. I had 100% germination success with their method. Once again a pretty small sample to mean much. I am very happy with the Hugo blocks and the huge nutrient savings with the drip irrigation system ~ 70% over DWC.