Bob's Journey into Hybridisation and Genetics - For Dummies

[Corum59]

Actually you can turn those lights around as to open the holes more or less. The lights themselves are only 5V, so danger of electrocution isn't a factor.

I was thinking more of moisture causing a short of the diodes and the connectors, but yeah 5v isnt going to be an issue and they ae cheap as chips anyway.

 

[Bob's Auto's]

Hey guys,

Sorry for my absence lately, but some sh!te has happened. Don't wanna talk about it so let's go on with the show
I'm calling this the end of Day one for my new Mephisto's Wedding Back crossed into regular form...
I named this cultivar Junk Dealer's Wedding.
It's heritage is made up from ♀ Mephisto's Wedding x ♂ Sooty Bride (♀ Mephisto's Wedding x ♂ Coal Miner's Daughter)
After selection, sixteen candidates remained to be planted, each inside a 3.5L pot.

I only got the pictures from the time lapse camera. These start from when I translplanted the seedlings from the germination boxes into their final resting place The speed = 30fps, duration = 23hours. I know, by the time I'm posting it on the forum, the 24 hours will have past, but hey...it takes time to process them timelapses... Oh, found this neat function on the camera that shows the time in the bottom right corner. I could also add the date, but I felt it was less important than showing the time...
Enjoy

Front left sorta... actually lower corner...

Back left corner.

Back right...yup, we're going around the clock...

Last one for today, I promise

I hope we're off to a good start this time


Regards,

Bob

 

[Bob's Auto's]

@DOA, thanks for the rep my friend

Day two today and even though it looks like the seedlings aren't doing much, the contrary is shown in the time lapses.
Let's start with the overview of the entire tent. Sorry for the crooked framing, but every time I close the tent the gorilla pod changes position slightly...

Anyway, duration = 24 hours, speed = 30 fps. Please enjoy

Regards,

Bob

 

[Bob's Auto's]

Small addition for those of you that are tech savvy...
I've upgraded the code on my ESP32 devices (environmental sensor and the fan controller) and now I'm able to keep my VPD stable between 0.6 and 0.7kPa. I added a new function in the Blynk app (marked by a circle, below) which checks the VPD value from the environmental sensor and when it's below 0.6kPa it runs the fan 1% faster. When the VPD gets to 0.7kPa it backs down the fan by 1%. Yes!, 1% is enough to get everything out of whack, that's why I've made this alteration. Either I ran the fan at 5%, but then the VPD was getting to high or I ran it at 4% and the VPD get's to low, like in the 0.4kPa...Which is fine if you've just sown the seeds, but I'm trying to get seedlings to grow, so I need the air to be dryer and get some transpiration going.

While I'm at it, let's show off some other values.
Temperature is a bit high, but everything else is in spec, so I choose to keep it at 27-28°C ánd value my VPD above everything else. As long as the humidity can keep up with the high temperature I'm golden.
The DIY CO² generator I made last season is still up and running. I think I made it to big for my setup, which is like everything else I'm running in this tent, But better to have some leeway, then having to run everything at 95% all the time...

I'm running the lights a bit higher as well, just to make sure the seedlings use up as much of the extra CO² I provided for them. Normally I would keep 'm at 12-16 Mole, but as everything is running a bit higher I choose to keep it at 18 Mole.

 

[JP1]

Fantastic thread here @Bob's Auto's Educational and inspirational

 

[Bob's Auto's]

Fantastic thread here @Bob's Auto's Educational and inspirational

Thanks brother

 

[Bob's Auto's]

Thanks for the rep @JP1 & @Antonio_DutchPassion

We're a couple of days on and the seedlings sure have taken off. Glad to see everything working out the way it should.
All these sprouts were the fastest to come out and from those only the largest got selected. In the meantime all other seeds had emerged and got fed to my pet hamster Actually set it asside as I didn't need the remaining seeds to emerge, but then this weekend I saw the germinationboxes on the potting table and lookie here...They all were looking for much needed light and stretching like hell.

Anyway, some close-up's for ya'll before I shower you with some time lapse goodies...

Just watered the soil as I had worked in some Mycotrex beforehand.

Next watering will probably not be for the next two days. I try to give as much water as the plant consumes in a 24 hour period, but giving slightly more and waiting 36-48 hours before watering again makes the roots go down in search of water...

...This also drives the roots into the higher nutrient rich soil and closer to the BioTab.

This is a fresh batch of soil, but I'm already satisfied by how it looks and responds to a water gift. The bennies inside are really starting to colonize the medium and that's why I added a new dose of Mycotrex before watering...now it's time for the myco to do it's thing and expand the rootsystem of the seedlings.

I'm also very satisfied with the addition of the code, which ables me to set a condition when to run the fan 1% higher. In the picture below, you'll see the situation just after the fan was turned down to 4%. It takes about an hour for the VPD to drop from 0.83kPa to 0.72kPa in which case the fan runs at 5% until the 0.82kPa is reached, then it overshoots by 0.03 or something and then slowly starts to drop again...
Next up the list to build is a humidifier which also runs on a D1 mini.

Ok you guys, the time lapses...I can only recommend you install a camera yourself as you'll learn quite a lot from the behaviour of your plants, albeit after the facts, but in time you gain quite some knowledge into adjusting the speed of a fan or your lights, just because your plants start reacting different. What I'm looking for is the dancing behaviour in the seedlings, as then growth is the fastest.
Have a look for yourselves...Duration = 68 hours, speed = 30fps.

And a couple zoomed in to a cluster of seedlings...

Regards,

Bob

 

[Bob's Auto's]

Hey friends


We're a week on and the seedlings are really enjoying their stay inside the tent, even though I am pushing them quite a bit, but more on that in a moment. It's almost time to give 'm a first kick in the bud with some Boom Boom Spray. This will be done at day ten, but I like to mention it now so I don't forget it in a day or two Until then it's dechlorinated water only. I might even delay watering until then, because I would also like to feed the bennies with some Orgatrex.

The parameters inside the tent are remarkable well if I may so myself...

• We're almost @ 20 Mole with the DLI, it's only three percent to get there, but I saw some reaction on the rims of the leaves, so I toned it down...We'll get there in a couple of days
• CO² is still at double the concentration compared to the outside and all thanks to this DIY yeast CO² generator I made last year. Anyway, it's free food for the plants so I'm happy to get it mixed inside the tent.
• The temperature is on the high side, but keeping the humidity higher, the VPD (and what's more important then that you guys?) is just right.

Because of the software upgrade I'm now able to adjust the fan on a setpoint (VPD of course) basis, which in turn enable's me to...relax. I haven't visited the tent in two days. I do still keep an eye on the girls via the time lapse camera, but I only go to the tent when the girls need a drink...Thinking of that, I still have more then enough solenoids lying around to rig something up...But then again, I wouldn't go there at all...No, let's keep those solenoids for the greenhouse.
Anyway...as I told you, I was pushing the girls...Like any of us, I want to get the most and best out of this cultivar. It is a test...a creation of myself, standing on the shoulders of more knowledgeable people. And I would like to select the best genetic donor's I can for this next generation. In the meantime I would also like to get me some killer bud to keep my jars full, so these girls will be pushed to the very edge when it comes to VPD, light, nutrition and stimulators.

Since it's been three days ago I posted an update, you guys are in for an extra long time lapse.
Speed = 30 fps, duration = 64 hours. The second halve of the time lapse(s) you'll notice a jump in activity. This is when I decided to push the VPD from an average of 0.70kPa to a more dryer environment of 0.80kPa. This ensures the girls need to work harder to keep up with evaporation and in doing so take up loads of nutrients.

Hope you enjoyed Today's show


Regards,

Bob

 

[Corum59]

Hey friends


We're a week on and the seedlings are really enjoying their stay inside the tent, even though I am pushing them quite a bit, but more on that in a moment. It's almost time to give 'm a first kick in the bud with some Boom Boom Spray. This will be done at day ten, but I like to mention it now so I don't forget it in a day or two Until then it's dechlorinated water only. I might even delay watering until then, because I would also like to feed the bennies with some Orgatrex.

The parameters inside the tent are remarkable well if I may so myself...

• We're almost @ 20 Mole with the DLI, it's only three percent to get there, but I saw some reaction on the rims of the leaves, so I toned it down...We'll get there in a couple of days
• CO² is still at double the concentration compared to the outside and all thanks to this DIY yeast CO² generator I made last year. Anyway, it's free food for the plants so I'm happy to get it mixed inside the tent.
• The temperature is on the high side, but keeping the humidity higher, the VPD (and what's more important then that you guys?) is just right.

View attachment 1633566

Because of the software upgrade I'm now able to adjust the fan on a setpoint (VPD of course) basis, which in turn enable's me to...relax. I haven't visited the tent in two days. I do still keep an eye on the girls via the time lapse camera, but I only go to the tent when the girls need a drink...Thinking of that, I still have more then enough solenoids lying around to rig something up...But then again, I wouldn't go there at all...No, let's keep those solenoids for the greenhouse.
Anyway...as I told you, I was pushing the girls...Like any of us, I want to get the most and best out of this cultivar. It is a test...a creation of myself, standing on the shoulders of more knowledgeable people. And I would like to select the best genetic donor's I can for this next generation. In the meantime I would also like to get me some killer bud to keep my jars full, so these girls will be pushed to the very edge when it comes to VPD, light, nutrition and stimulators.
View attachment 1633567


Since it's been three days ago I posted an update, you guys are in for an extra long time lapse.
Speed = 30 fps, duration = 64 hours. The second halve of the time lapse(s) you'll notice a jump in activity. This is when I decided to push the VPD from an average of 0.70kPa to a more dryer environment of 0.80kPa. This ensures the girls need to work harder to keep up with evaporation and in doing so take up loads of nutrients.

View attachment 1633578

View attachment 1633579

View attachment 1633580

View attachment 1633581

View attachment 1633582


Hope you enjoyed Today's show


Regards,

Bob

Gotta love a few good time lapse videos, seeing life spedup really gives one a good take on what actually happens in there.

 

[Bob's Auto's]

Gotta love a few good time lapse videos, seeing life spedup really gives one a good take on what actually happens in there.

That's so true my friend. What I would really love to do is add a graph with the current values measured in my Blynk app. I know it's possible and I do have the possibility to extract a csv file from the app. Just a lack of knowledge on my part.
It would be cool though to see exactly how a plant responds to a certain VPD value for instance. What I do now is lookup a period when some value is stable and then compile that piece like I do when making a time lapse for the forum. It's quite some work, but you learn so much from it...
You know what...I'm gonna see if I can't find any youtube clip to help me out...There's nothing you can't find on Google