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The nematodes do not die off they may go dormant but as long as the soil remains moist they will live and reproduce. The life cycle consists of 6 stages. Nematodes are present in the soil at all stages at the same time. Drying out or freezing will kill all stages.How so? The nematodes die off once pest are dead so at minimum seasonal application is required.
The life cycle of beneficial nematodes consists of eggs, four larval stages and the adults. The third larval stage is the infective form of the nematode (IT). They search out susceptible hosts, primarily insect larvae, by detecting excretory products, carbon dioxide and temperature changes.