Bob's Journey into Hybridisation and Genetics - For Dummies

[Bill.de.Cat]

Correct my friend
Next season I'm gonna dip my tows in to Polyploidy and see what we can make...or not

Some of what I read placed germ rates as low as 40% after treating, if you got plenty of seeds it could be worth it..

 

[WildBill]

Some of what I read placed germ rates as low as 40% after treating, if you got plenty of seeds it could be worth it..

What does it do?

 

[kfigerm]

HA..favourable...hope so, atm it looks odd yellow, but she recovers...time will tell if it's favorable..if I understood correctly this is one (whith three leaves).


but now I wonder if i should put her out of the grow and do try pollen with cs on her? Worth it? Or let grow out as a girl?
But i read some about it on the internet, and I understand, that breeders go for that. So now I wonder, is this just an accident or is it something going on @Antonio_DutchPassion in this direction? And whats the experts view on that?

 

[Bill.de.Cat]

What does it do?

Can induce polyploidy in plants.
edit: Polyploidy is the condition of multiple sets of chromosomes within one cell. Cannabis has 20 chromosomes in the vegetative diploid (2n) condition. Triploid (3n) and tetraploid (4n) individuals have three or four sets of chromosomes and are termed polyploids. It is believed that the haploid condition of 10 chromosomes was likely derived by reduction from a higher (polyploid) ancestral number (Lewis, W. H. 1980). Polyploidy has not been shown to occur naturally in Cannabis; however, it may be induced artificially with colchicine treatments. Colchicine is a poisonous compound extracted from the roots of certain Colchicum species; it inhibits chromosome segregation to daughter cells and cell wall formation, resulting in larger than average daughter cells with multiple chromosome sets

 

[Bob's Auto's]

Some of what I read placed germ rates as low as 40% after treating, if you got plenty of seeds it could be worth it..

I know that's why I was thinking in doing it next season. If I'm doing everything right in my tent, the males should be making viable pollen as we speak. The females in waiting shall be ready soon and hopefully provide me with ample material to start with.

What does it do?

It induces Polyploids in cannabis seeds/sprouts.

 

[Bill.de.Cat]

I know that's why I was thinking in doing it next season. If I'm doing everything right in my tent, the males should be making viable pollen as we speak. The females in waiting shall be ready soon and hopefully provide me with ample material to start with.


It induces Polyploids in cannabis seeds/sprouts.

Haven't read anything yet that leads me to believe the trait carries on through succeeding generations though....
edit: Once again, little emphasis has been placed on the relationship between flower or resin production and polyploidy. Further research to determine the effect of polyploidy on these and other economically valuable traits of Cannabis is needed.
and there's always that....since better buds are what we are looking for.

 

[Bob's Auto's]

Haven't read anything yet that leads me to believe the trait carries on through succeeding generations though....
edit: Once again, little emphasis has been placed on the relationship between flower or resin production and polyploidy. Further research to determine the effect of polyploidy on these and other economically valuable traits of Cannabis is needed.
and there's always that....since better buds are what we are looking for.

From what I've read, (LINK) it's the Tetraploids we're after. If you cross a tetraploid with a diploid (standard seed), you get a triploid. Now those can only be bred with other triploids. So we want to cross a tetraploid with a tetraploid to keep the extra chromosomes inside the plants. It's these chromosomes that can activate certain traits like vigor, size, amount of flowers, and so on. By adding two extra chromosomes you create 12 times more possibilities to activate a trait. ie, two x two (chromosomes) = 4 possible combinations, but four x four = 16 combinations...exponentially expanding possible traits and even new traits which express after a combination of two alleles from different chromosomes exist in the offspring's single chromosome creating a mutation... The fact is, without the necessary lab equipment you don't know if you're working with a tetra-, hexa- or other polyploid. But the fact you can create something new, a mutation that creates a new strain like the old-timers did in the 80-90's is so appealing.

 

[Bob's Auto's]

Feck this Covid... one moment you feel as if you have been through the worst and get a breather. The next I'm gasping for air... Did manage to get to the greenhouse and water the plants, also made a visit to the tent to check the males, water them, apply STS and take some pictures

This one is the one I broke and resides inside a glass of water so I'm not expecting to much from this 'male'

The Purlple Kush seems to have pushed some pollen already, guess I was to late for this first wave, but as you can see there are still pollen sacs which have to open. Now every male got an aluminum skirt which should trap the pollen from each plant. The monkey fan isn't running and the extraction fan is set to it's minimum. I've turned the lights down to 25% which is dim, but lowers the DLI and should give a signal to the plant that 'winter is coming'

So...All males are showing balls and this shows' you don't have to be a rocket scientist to create males with STS

Hope you enjoyed todays post(s)


Regards,

Bob

 

[Bill.de.Cat]

From what I've read, (LINK) it's the Tetraploids we're after. If you cross a tetraploid with a diploid (standard seed), you get a triploid. Now those can only be bred with other triploids. So we want to cross a tetraploid with a tetraploid to keep the extra chromosomes inside the plants. It's these chromosomes that can activate certain traits like vigor, size, amount of flowers, and so on. By adding two extra chromosomes you create 12 times more possibilities to activate a trait. ie, two x two (chromosomes) = 4 possible combinations, but four x four = 16 combinations...exponentially expanding possible traits and even new traits which express after a combination of two alleles from different chromosomes exist in the offspring's single chromosome creating a mutation... The fact is, without the necessary lab equipment you don't know if you're working with a tetra-, hexa- or other polyploid. But the fact you can create something new, a mutation that creates a new strain like the old-timers did in the 80-90's is so appealing.

Bringing back high school memories....

 

[Bill.de.Cat]

Feck this Covid... one moment you feel as if you have been through the worst and get a breather. The next I'm gasping for air..

I hear ya...breathing hasn't been the same since wife and I got a mild case.

 

[Bob's Auto's]

I hear ya...breathing hasn't been the same since wife and I got a mild case.

Damn, that's sad to hear brother. Hope it recovers in the future for the both of ya
I've slept almost the entire day and of course I can't sleep now. But, luckily I have the TV all to myself and let's not forget you guys...