That's what I did recently as per your suggestion and I've had zero sterile procedure related contaminations since. I can wholeheartedly recommend this. It does make a huge difference and has given me a complete peace of mind. Thanks, by the way!
But as RogerRabbit once wrote: "Once your spawn jars are fully colonized, you can scratch your butt while you inoculate the coir if you want."
If the spawn is produced in sterile enough conditions and the secondary substrate properly pasteurized, clean room or no the tub should be 100% successful.
Can you describe how you made the tea? I doubt the maturity of the fruits affects potency that much. You should just have an increased water to alkaloid ratio.
Be sure to boil the shit out of it. The degradation of the active compounds is hugely overrated and in truth you won't see much loss in potency at 100 celsius. In this case it does make water more efficient as a solvent, though.
I've heard mixed reviews on re-dosing psychedelics since a tolerance is built pretty much right after consuming the first dose. That might explain the underwhelming second dose. I've also found my trips to be less and less magical when I do them every two weeks. Maybe give it some time? That's what I'm forced to do now
In any case, contaminations shouldn't alter the fruits' properties in any discernible way.
Second tub is looking amazing!