S1 or ???

[DarKnight]

I started with 30 mephisto toothless alien auto fem, reversed 2, bred them to remaining 28. Not sure if that makes a difference...

Parental generation absolutely makes a difference. You have made an R1 cross. Toofless Alien is a limited edition Feminized R1 hybrid between Ripley's OG and Toof Decay.

An F1 can be very much true to form. It depends of course on how homozygous the plants used in the breeding were. This is how many big seed vendors put out f1 seeds each year that breed the same phenotype. An R1 cross, similarly made with reversed females will follow the same rules. It all depends of course on how homozygous or true breeding the parental stock is.

Crossing two of the same f1 together gives you an f2 or filial generation 2. The filial generation 2 is nowhere near as stable as the f1 was and that is why large grow outs are done with the f2 and beyond. A large grow out allows you to hunt for the phenotypes you are trying to marry between the two parental plants. A triple recessive trait would have only a 1/64 chance of popping up. Which is why you have to grow out large numbers of those f2. An example of f1 x f1 =f2 would be (C. sativa 'Panama Red' x C. ruderalis) x (C. sativa 'Panama Red' x C. ruderalis) if you used siblings.

Crossing two different R1 plants together from the same cross as you have done would give you an R2 as your cross is (Toofless Alien x Toofless Alien) you have however started to create 60 lines if you simply get 1 seed from each pairing to result and you have kept track of the pairings. With 20 chromosomes 1,048,576 possibilities exist without including chromosome crossover.

The more seed you can plant and the higher your filial generation or "R" generation the more you are able to hone in on the phenotype you are selecting for provided you are growing out many seeds to see which lines are producing the phenotype you are going after.


This is exactly how breeders create stable true breeding plants, but it takes time, generations, and numbers. Then you can talk about how your line is able to produce the desired phenotype 90% of the time out of grows of 100 seedlings each generation or 95% of the time out of 200 seedlings planted.

 

[Blu_tri]

let’s say a bird poops a single cannabis seed in an isolated area. When the proper conditions were met, the seed hatched a seedling.
Now let’s say over the next few months the seedling turned into a beautiful female cannabis plant.
what happens next is indicative to most plant species. The female matures and there was no male around. This female produced her own pollen and make seed with herself.
The seed fall and produce new seedling for the next generation. Theoretically these seed should be female since they come from self pollination.
It’s the next season and once again proper conditions are met, but this time there are hundreds of seeds. Also this time one seed produces a male. Therefore the next generations contain both mail snd female plants.
Who knows how long this cycle has been repeated by the time the strain has been found by man. But when man does get there, he finds male and female plants. The thought never occurred to the finders of the population, that his find started from a single cannabis seed.
Food for thought.