Cloning Autoflowers

[Jimmythenugg]

Hey @Son of Hobbes Omelette du fromage?

 

[Son of Hobbes]

Hey @Son of Hobbes Omelette du fromage?

Add ham, onions, and green peppers and we may be talking.

 

[Death The Cultivator]

The problem with that is "who has an environment suitable for tissue cloning." Most commercial grows likely wouldn't even be able to accommodate this without spending a chunk of coin. And for joe-home-grower? Even less so.

What we need is someone on here who happens to have a lab in their basement. Bonus points if your name is Dexter.

The shroom growers with laminar flow hoods on here have the best chance lol

 

[Anthropolis]

Or at minimum, like was mentioned, a DIY glove work box and a good knowledge of sterile practices could probably get some TC doable for a home grower. I did a bit of shroom work years ago and it's probably within reach. I know shroom TC replication just needs agar petri dishes. I think plant TC cloning takes a good bit more labor and some hormones and gear, but should still be performable in a glove box... in theory. lol.

It would really be an awesome savings in auto seeds and pheno hunting. Say you wanted a bunch of guaranteed purple bud kush as a final product. You could spend a few hundred on 25 seeds, probably have 1/3 pheno all dark purple. With TC you could theoretically buy just a 5 pack, start em and take 20 TC of each early, then just grow out the TC's of the pheno you want. By then one does ask themselves, why not just run photoperiod clones lol.

 

[Groff]

Or at minimum, like was mentioned, a DIY glove work box and a good knowledge of sterile practices could probably get some TC doable for a home grower. I did a bit of shroom work years ago and it's probably within reach. I know shroom TC replication just needs agar petri dishes. I think plant TC cloning takes a good bit more labor and some hormones and gear, but should still be performable in a glove box... in theory. lol.

It would really be an awesome savings in auto seeds and pheno hunting. Say you wanted a bunch of guaranteed purple bud kush as a final product. You could spend a few hundred on 25 seeds, probably have 1/3 pheno all dark purple. With TC you could theoretically buy just a 5 pack, start em and take 20 TC of each early, then just grow out the TC's of the pheno you want. By then one does ask themselves, why not just run photoperiod clones lol.

I dont think it is possible to isolate phenotypes from TC, as this is primarily an environmental factor.
However, cloning into the same environment will do the trick.
_

 

[Death The Cultivator]

Or at minimum, like was mentioned, a DIY glove work box and a good knowledge of sterile practices could probably get some TC doable for a home grower. I did a bit of shroom work years ago and it's probably within reach. I know shroom TC replication just needs agar petri dishes. I think plant TC cloning takes a good bit more labor and some hormones and gear, but should still be performable in a glove box... in theory. lol.

It would really be an awesome savings in auto seeds and pheno hunting. Say you wanted a bunch of guaranteed purple bud kush as a final product. You could spend a few hundred on 25 seeds, probably have 1/3 pheno all dark purple. With TC you could theoretically buy just a 5 pack, start em and take 20 TC of each early, then just grow out the TC's of the pheno you want. By then one does ask themselves, why not just run photoperiod clones lol.

I'm still interested in doing TC but I can't guarantee the sterility without a laminar flow hood cause I mess up frequently lol. There were few people trying to build home and travel TC kits but never went anywhere.

Hemp Multiplication Kit

The purpose of this kit is to demonstrate in vitro vegetative propagation. This kit provides the necessary materials to initiate cultures from the seeds and/or stem tissues of hemp. Plant tissues are not included in kit and must be supplied by the user.

phytotechlab.com

That is the kit I'm still looking at. They provide full instructions, measurements, etc. Might be worth it for someone with a library of genetics or the desire for clean clones.

 

[Son of Hobbes]

I'm still interested in doing TC but I can't guarantee the sterility without a laminar flow hood cause I mess up frequently lol. There were few people trying to build home and travel TC kits but never went anywhere.

Hemp Multiplication Kit

The purpose of this kit is to demonstrate in vitro vegetative propagation. This kit provides the necessary materials to initiate cultures from the seeds and/or stem tissues of hemp. Plant tissues are not included in kit and must be supplied by the user.

phytotechlab.com

That is the kit I'm still looking at. They provide full instructions, measurements, etc. Might be worth it for someone with a library of genetics or the desire for clean clones.

Hmmm. Interesting link. I wonder if this company would be interested in sending a few kits to up-and-coming autoflower tissue culture enthusiasts.

 

[pop22]

But one tissue culture can produces dozens of clone and they can be stored for months. Tissue culture is beginning to be adopted by the Cannabis,
( Ohh how I hate this word.. ) industry.. They can store thousands of clones in the space taken up by a couple of trays of cuttings. And there are kits for home growers who'd like to try. They use stem tissue though not leaves, although I don't see why they couldn't be used as leaf tissue. I believe you need a tool though to slice thin layers of a leaf.

kits here:

http://kitchenculturekit.com/

Or at minimum, like was mentioned, a DIY glove work box and a good knowledge of sterile practices could probably get some TC doable for a home grower. I did a bit of shroom work years ago and it's probably within reach. I know shroom TC replication just needs agar petri dishes. I think plant TC cloning takes a good bit more labor and some hormones and gear, but should still be performable in a glove box... in theory. lol.

It would really be an awesome savings in auto seeds and pheno hunting. Say you wanted a bunch of guaranteed purple bud kush as a final product. You could spend a few hundred on 25 seeds, probably have 1/3 pheno all dark purple. With TC you could theoretically buy just a 5 pack, start em and take 20 TC of each early, then just grow out the TC's of the pheno you want. By then one does ask themselves, why not just run photoperiod clones lol.

 

[pop22]

YES!

Hmmm. Interesting link. I wonder if this company would be interested in sending a few kits to up-and-coming autoflower tissue culture enthusiasts.

 

[Kinezokyprios]

Since tissue culture is very expensive even for most of the commercial growers, it is good to get practical information on it but the focus should be on low cost small scale experiments that can be done by anyone at home.
When it comes to autos, cloning is worth trying in order to get seeds especially feminised ones, but practice proves it as unsuccessful in increasing bud yields. However the potential for seed production is more than worth exploiting. A tiny bud (a few of them to be precise) can produce enough seeds for a quite big grow op or for many years of small home grows. Plus you can even experiment creating your own strains without wasting your main crop.