Bob's Journey into Hybridisation and Genetics - For Dummies

[Antonio_DutchPassion]

Looking good mate. Some nice buds appearing all over. Sweeeeeeeeeet.

You were up early mate ;-) or still awake?

 

[Bob's Auto's]

You were up early mate ;-) or still awake?

Early brother, am at work now

 

[Bob's Auto's]

Hey breeders and wannabreeders


After a week of drying inside my study, I decided the packet with the flowers was good to carefully be pulled apart and slowly but carefully, extract all the collected pollen from it...And there was loads of pollen in there. So glad with this little harvest, which almost took me an hour start to finish.

I spread out some printer paper and carefully tapped the aluminum foil so the pollen would drop. To hard a tap and the pollen took to the air
Afterwards I removed both the spent and unripe flowers and carefully transferred the pollen into the small vial.
This is about a hundred times more pollen then I had before, so I'm really glad I started to play with regular auto seeds.

Next on the list will be to polinate the ladies in waiting. Oh and let's not forget a branch on that front Mephisto Wedding. Doing an actual Bx with the aid of the Mephisto Wedding x CMD pollen will give me something nice to play with in the colder months. Might even do a SOG to see what differences show up.
Not wanting to call it a pheno hunt as sixteen plants can barely be called a pheno hunt, but you know what I mean, right?

Question for the more advanced breeders...should I add oven baked flour to the pollen, don't really need to, as I most likely have more then enough pollen to sex up an entire field. But rather to help in preserving it for storage inside the freezer. Or is it better to leave it alone. It is very dry as humidity is at an all time low of 25% because of the hot weather. And the small vial has a small plastic cap plugged in the opening before the black cap went on it.
The plan is to keep the leftover pollen inside the vial in a metal thermos flask. You never know if for one reason or another I have need for this pollen again...in the future and I would like it to keep for as long as posible.

 

[Bob's Auto's]

Hey you guys


We've had a partially cloudy day today and the plants got a needed break from the continuous bombardment of sunshine.
Still temperatures got up to 36°C inside...Good for the PK Booster Compost tea, but the plants prefer a more moderate 26-28°C...Especially when your receding inside a 3.5L pot...

One of those is this lady in waiting...The Double Grape x Toof Decay. Looking nice and frosty already. Wondering how long to wait to uptimally pollinate her. I'm going to do a double pollination with a two day pauze inbetween, just like @Sawney_bean advises. Just to optimze the amount of seed setting.

Nice and frosty buds she has...

Next up we have the Mephisto's Wedding x Coal Miner's Daughter. This one is one hell of a grower!! Healthy looking leaves and a bit of Nitrogen hungry, but I've remediated that by mixing in some Silicium Flash in the water I fill the tray with. Tomorrow they will also receive a healthy dose of the tea. So tonight, I just gave them some water

She's full with pistils everywhere you look. And you wait until I show off her big sister...Wawawie...

Last lady in waiting is the Mephisto's Wedding x Forum Stomper. I truly forgot to select the best of the two and transplant it into a bigger pot.

A real shame as she's producing copious amounts of trichomes and looking at it, rockhard buds. Stil going to pollinate the large one and keep the small one to have something to test out.

Those where the ladies inside the small 3.5L pots. We now move on to the one's that got selected for transfer into the bigger 7 and 10 gallon pots.


First off, the Double Grape x Toof Decay. This lady isn't looking bad, but I expected her to fill out more. Instead she's behaving like a spoiled cheerleader who doesn't want to eat because it's making her look fat. No, this has more to do with the amount Sativa in her blood then anything else. A typical Sativa trade...Large internodal distancing, Long slender leaves and pockets of bud sites.

She is full on into flowering and anyone may know about it. Pumping out pistils like crazy!!

Next up we have a monstrous cola in the making. This Mephisto's Wedding x CMD cross is really kicking some ass!!
What do you think @Sawney_bean? In a smaller setting this could be an amazing SOG cultivar, no?

And the calyx's haven't even swollen up yet!! Lol, this will be something to look out for...Let's keep our the humidity will stay as low as it's been the last few weeks. But, have a feeling I've got it under control with the automated waterings. This way, fewer water will be spilled onto the floor, which doesn't have to evaporate and raise the humidity...Action and consequence

The amount of frost on this girl is just....Mhmmm....

Healthy looking leaves, pistils standing erect...

And finally we have our two Mephisto's Wedding sisters. The first one is definitely a class-A specimen, but her sister is kinda mediocre. I'm even pondering to cull her. The only reason I'm thinking otherwise is...maybe she'll produce something special after all...Which I've been reading about, but I don't know if I want to give this one 100+ days to get there...Really want to finish by the end of July so I can go on holiday without having to look over my shoulder the whole time...

But this first lady though...O boy, have a look at the amount of frost she is showing!!

 

[Bob's Auto's]

A real prime specimen, don't you think so?

Can't wait to test this one out. Can't forget to pollinate one branch on this one as well...
Oh well, and with this we've come to an end for today's (actually yesterday's post by now) post. I wish you guys a fine day/evening/night and see you next time.

Oh, yes...Question, looking at the picture's from the ladies in waiting you guys, when should I start pollinating? Can I have a go or should I wait until more pistils are showing? Can remember reading something about waiting until the first brown pistil is showing, but that will be a couple more days or weeks, no?


Regards,

Bob

 

[FarmerJake]

Oh, yes...Question, looking at the picture's from the ladies in waiting you guys, when should I start pollinating? Can I have a go or should I wait until more pistils are showing? Can remember reading something about waiting until the first brown pistil is showing, but that will be a couple more days or weeks, no?

You are correct that Sawney reccomended waiting to see those first pistils mature out before pollinating. Still every time I have done it up to now I would be chucking pollen at them ASAP because I’m always nervous I’ll probably kill the plant before they mature if I don’t Get that 6 weeks seed maturing window started right? But I think for best quality follow the pros and listen to Sawney. Your husbandry is on point to carry them through regardless

 

[Rasterman]

Hey you guys


We've had a partially cloudy day today and the plants got a needed break from the continuous bombardment of sunshine.
Still temperatures got up to 36°C inside...Good for the PK Booster Compost tea, but the plants prefer a more moderate 26-28°C...Especially when your receding inside a 3.5L pot...

One of those is this lady in waiting...The Double Grape x Toof Decay. Looking nice and frosty already. Wondering how long to wait to uptimally pollinate her. I'm going to do a double pollination with a two day pauze inbetween, just like @Sawney_bean advises. Just to optimze the amount of seed setting.
View attachment 1604947
Nice and frosty buds she has...
View attachment 1604948

Next up we have the Mephisto's Wedding x Coal Miner's Daughter. This one is one hell of a grower!! Healthy looking leaves and a bit of Nitrogen hungry, but I've remediated that by mixing in some Silicium Flash in the water I fill the tray with. Tomorrow they will also receive a healthy dose of the tea. So tonight, I just gave them some water
View attachment 1604949

She's full with pistils everywhere you look. And you wait until I show off her big sister...Wawawie...
View attachment 1604951

Last lady in waiting is the Mephisto's Wedding x Forum Stomper. I truly forgot to select the best of the two and transplant it into a bigger pot.
View attachment 1604952

A real shame as she's producing copious amounts of trichomes and looking at it, rockhard buds. Stil going to pollinate the large one and keep the small one to have something to test out.
View attachment 1604954


Those where the ladies inside the small 3.5L pots. We now move on to the one's that got selected for transfer into the bigger 7 and 10 gallon pots.


First off, the Double Grape x Toof Decay. This lady isn't looking bad, but I expected her to fill out more. Instead she's behaving like a spoiled cheerleader who doesn't want to eat because it's making her look fat. No, this has more to do with the amount Sativa in her blood then anything else. A typical Sativa trade...Large internodal distancing, Long slender leaves and pockets of bud sites.
View attachment 1604955

She is full on into flowering and anyone may know about it. Pumping out pistils like crazy!!
View attachment 1604958
View attachment 1604959

Next up we have a monstrous cola in the making. This Mephisto's Wedding x CMD cross is really kicking some ass!!
What do you think @Sawney_bean? In a smaller setting this could be an amazing SOG cultivar, no?
View attachment 1604964

And the calyx's haven't even swollen up yet!! Lol, this will be something to look out for...Let's keep our the humidity will stay as low as it's been the last few weeks. But, have a feeling I've got it under control with the automated waterings. This way, fewer water will be spilled onto the floor, which doesn't have to evaporate hand raise the humidity...Action and consequence
View attachment 1604969

The amount of frost on this girl is just....Mhmmm....
View attachment 1604970

Healthy looking leaves, pistils standing erect...
View attachment 1604972


And finally we have our two Mephisto's Wedding sisters. The first one is definitely a class-A specimen, but her sister is kinda mediocre. I'm even pondering to cull her. The only reason I'm thinking otherwise is...maybe she'll produce something special after all...Which I've been reading about, but I don't know if I want to give this one 100+ days to get there...Really want to finish by the end of July so I can go on holiday without having to look behind my shoulder whole of the time...
View attachment 1604973

But this first lady though...O boy, have a look at the amount of frost she is showing!!
View attachment 1604974

That Double Grape X Toof Decay is going to fill all that internodal space with buds.

 

[Bob's Auto's]

You are correct that Sawney reccomended waiting to see those first pistils mature out before pollinating. Still every time I have done it up to now I would be chucking pollen at them ASAP because I’m always nervous I’ll probably kill the plant before they mature if I don’t Get that 6 weeks seed maturing window started right? But I think for best quality follow the pros and listen to Sawney. Your husbandry is on point to carry them through regardless

Thanks for the heads-up mate!
Checked today and saw some pistils which had started shriveling. So I'm going to be looking at them every day and make my decision at that time.

That Double Grape X Toof Decay is going to fill all that internodal space with buds.

I really hope so bro.
Can you tell me something about it? Couldn't find anything on this cross. Have been looking on google and redit to see if I could find the correct Illuminauto n°, but alas.
With the aid of VPN, I was able to read up on the parents on Mephisto's website and both have definitely got the things I like in 'm. Combine the fact that it's probably a F1 cross, hybrid vigor should be doing it's thing.



This morning I disabled the automatic watering system and manually drenched the pots with the diluted tea. Each pot got about 3L and the big pot got an entire watering can (5L) of the stuff.
Tonight after sundown I drenched the plants with another 2L Boom Boom Spray. I start on the underside of the leaves and make sure I get into every nook and cranny. Then with the remainder I treat the topside of the leaves. I always have some left over, so this is never wasted and gets sprayed on my other plants inside the greenhouse.

 

[Rasterman]

I really hope so bro.
Can you tell me something about it? Couldn't find anything on this cross. Have been looking on google and redit to see if I could find the correct Illuminauto n°, but alas.

Sorry, no. I haven't grown that strain. Long internodes are odd for either parent, but Double Grape does have sort of a sativa-leaning structure and if it's taking its cue from its Double Grape parent, I'm thinking it will fill in. I'm tuned in too, because I'm curious!

 

[Rasterman]

If you want to see internodes that won't be filling in....

(Apologies for the drop shadow.)