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Cannabinoids and floral maturity; terpenes and fatty-acid rancidification
It is well understood that cannabinoids are predominantly, if not entirely, synthesised and sequestered in small, globular structures called glandular trichomes. Most of the aromatic monoterpenes and sesquiterpenes found in the floral mass are also located in these structures. However, the gene activities involved in the bio-synthesis of cannabinoids remain unclear. The major chemical precursor of the synthetic pathway arises from the action of enzymes responsible for the control of each individual mechanism. It is generally accepted that drug varieties of cannabis have enzyme systems the convert CBD-acid to THC-acid and thereby favor THC-acid accumulation and that fiber strains of cannabis have been found to lack such enzymes. THC is the primary psychoactive substance secreted by glandular trichomes with CBN, CBD and other accessory canabinoids. It is reasonable to suspect therefore, that the most productive botanical material would contain these glandular trichomes in substantial quantities, and at their optimum stage of development (Potter 2004).
Blue Himalayan Kush in full bloom (wk8-9)
Fox-tail flower clusters - Blue Himalayan Kush (wk9)
Terpenes and other aromatic compounds may also serve to potentiate or suppress the effects of THC. We know that canabinoid levels may be used to establish canabinoid pheno-types and that these pheno-types are passed on from parent to offspring - therefore canabinoid levels are in the most part determined by genes (Clarke 1993).
Bluematic in full bloom (wk9) -left. Rancidified capitate-stalked trichome 400x magnification -right
The taste and aroma of cannabis essential oils is produced by the activity of nearly 20 different aromatic terpenes which are secreted as a component of the resin released from the glandular trichomes. When the floral clusters are squeezed by touch, the resin glands rupture exposing these terpenes to the air leading to their oxidation to derivatives. Polymerization of the aromatic terpenes leads to the accumulation of resinous solids as the flora material ages. Additional aromas may interfere with the terpinoid components such as ammonia and various other gaseous products released by the process of curing and fermentation.
Pineapple express in full bloom (wk9)
Several types of trichome are identifiable at peak maturity. The majority of the trichomes present resemble secretory glands either balbous, capitate and stalked. The capitate trichome is the principal form responsible for the intense secreation of cannabinoid laden oils. Therefore it is key to the breeder of drug varieties to select parent pheno-types with a high density of capitate stalked trichomes.
Pineapple express - translucent capitate trichrome (200x magnification)
The quality and quantity of resinous production varies according to variety. For instance, a strain may produce many trichomes but not secrete so much resin. Although in the early stages of flower development, the resinous secretions appear translucent, oxidative rancidification of trichome fatty acids leads to a change in their appearance and aroma; chemical decomposition of the translucent resin gives it an opaque or 'cloudy' appearance.
Bluematic in full bloom (wk8 -left, wk9 -right)
These changes are generally indicative of flower maturity. Some varieties however produce a blonde or translucent amber resin throughout flower development regardless and these indeed are often considered the most psychoactive species.
Bluematic (wk9) mature or 'amber' capitate trichomes (200x)
Smurfberry (wk9)
It is well understood that cannabinoids are predominantly, if not entirely, synthesised and sequestered in small, globular structures called glandular trichomes. Most of the aromatic monoterpenes and sesquiterpenes found in the floral mass are also located in these structures. However, the gene activities involved in the bio-synthesis of cannabinoids remain unclear. The major chemical precursor of the synthetic pathway arises from the action of enzymes responsible for the control of each individual mechanism. It is generally accepted that drug varieties of cannabis have enzyme systems the convert CBD-acid to THC-acid and thereby favor THC-acid accumulation and that fiber strains of cannabis have been found to lack such enzymes. THC is the primary psychoactive substance secreted by glandular trichomes with CBN, CBD and other accessory canabinoids. It is reasonable to suspect therefore, that the most productive botanical material would contain these glandular trichomes in substantial quantities, and at their optimum stage of development (Potter 2004).
Blue Himalayan Kush in full bloom (wk8-9)
Fox-tail flower clusters - Blue Himalayan Kush (wk9)
Terpenes and other aromatic compounds may also serve to potentiate or suppress the effects of THC. We know that canabinoid levels may be used to establish canabinoid pheno-types and that these pheno-types are passed on from parent to offspring - therefore canabinoid levels are in the most part determined by genes (Clarke 1993).
Bluematic in full bloom (wk9) -left. Rancidified capitate-stalked trichome 400x magnification -right
The taste and aroma of cannabis essential oils is produced by the activity of nearly 20 different aromatic terpenes which are secreted as a component of the resin released from the glandular trichomes. When the floral clusters are squeezed by touch, the resin glands rupture exposing these terpenes to the air leading to their oxidation to derivatives. Polymerization of the aromatic terpenes leads to the accumulation of resinous solids as the flora material ages. Additional aromas may interfere with the terpinoid components such as ammonia and various other gaseous products released by the process of curing and fermentation.
Pineapple express in full bloom (wk9)
Several types of trichome are identifiable at peak maturity. The majority of the trichomes present resemble secretory glands either balbous, capitate and stalked. The capitate trichome is the principal form responsible for the intense secreation of cannabinoid laden oils. Therefore it is key to the breeder of drug varieties to select parent pheno-types with a high density of capitate stalked trichomes.
Pineapple express - translucent capitate trichrome (200x magnification)
The quality and quantity of resinous production varies according to variety. For instance, a strain may produce many trichomes but not secrete so much resin. Although in the early stages of flower development, the resinous secretions appear translucent, oxidative rancidification of trichome fatty acids leads to a change in their appearance and aroma; chemical decomposition of the translucent resin gives it an opaque or 'cloudy' appearance.
Bluematic in full bloom (wk8 -left, wk9 -right)
These changes are generally indicative of flower maturity. Some varieties however produce a blonde or translucent amber resin throughout flower development regardless and these indeed are often considered the most psychoactive species.
Bluematic (wk9) mature or 'amber' capitate trichomes (200x)
Smurfberry (wk9)
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