Gnome Automatics My Journey to ALF#5

[mohawk warrior]

I'm game to ex[eriment, but it will have to wait till I can get the greenhouse going. I'll have space for aq few pots out there. Tents are mostly booked for about 2 years........

However, I see smaller pots not shortening flowering time. I think below 1 gallon, there is a limit to the soonest it will flower. I too have suspected larger pots will flower later when the root mass hits critical size. This test sounds interesting. A lot of possible variables though also. Strains may vary. Would be great, though likely not feasible, if we all grew the same strain.

With what I’ve been playing around with this seems true,
I’ve had something take forever same thing same time, in a solo cup life is quick
I believe the curling of the roots upwards send a biological trigger to the plant
I have plenty of room... but not a sufficiently adequate setting lmao...
Clones would be best... for science
Now that I said that..
Who’s to say the butchering of said limb did create a mutation heheheh... therefore tissue cultivation would be prime....
Now that I said that...
Would tissue culture from auto revert to its photoperiod genes only? Or does it retain the auto trait...

 

[pop22]

pot size certainly affects branch growth also. Here's a Blue Dragon in a 4" pot. In a 1 one gallon I get 8 branches, in a 3.9 gallon autopots, I get a lot of branches!

Here's my 9" Blue Dragon budsicle. The firstpic is her, no pruning done ever.

 

[pop22]

I still have supplies from my first attempt at tissue culture. I'll set it up again as soon as I can and see. I'm very curious as to the outcome of autoflower tissue culture!

With what I’ve been playing around with this seems true,
I’ve had something take forever same thing same time, in a solo cup life is quick
I believe the curling of the roots upwards send a biological trigger to the plant
I have plenty of room... but not a sufficiently adequate setting lmao...
Clones would be best... for science
Now that I said that..
Who’s to say the butchering of said limb did create a mutation heheheh... therefore tissue cultivation would be prime....
Now that I said that...
Would tissue culture from auto revert to its photoperiod genes only? Or does it retain the auto trait...

 

[Eclectic Elle]

I still have supplies from my first attempt at tissue culture. I'll set it up again as soon as I can and see. I'm very curious as to the outcome of autoflower tissue culture!

I'm intrigued. Tissue culture does what... grows a new plant from a small sliver, or something?

 

[mohawk warrior]

I'm intrigued. Tissue culture does what... grows a new plant from a small sliver, or something?

Ummmmmm....
Cloning can net let’s say 10 decent clones of a branch in a quick turnover with high probability of genetic duplication... while tissue culture cultivation can give supposedly exact DNA replication with a hell of a lot more time and work... a leaf could net 50 plus cultures
Lmao
Mind you better starting culture means quicker finish and such
... multiple stages with varying lengths, where a clone is like hey there’s roots transplant it

Then there’s contagions and stuff which can possibly alter shit and stuff things up

 

[Rev. Green Genes]

I'm intrigued. Tissue culture does what... grows a new plant from a small sliver, or something?

Tissue culture involves growing some cells (callose) on a petri dish and then inducing roots and or shoots from it with hormones. I have done it once, but it's been a minute. It all has to be quite sterile too.

 

[Rev. Green Genes]

I'm game to ex[eriment, but it will have to wait till I can get the greenhouse going. I'll have space for aq few pots out there. Tents are mostly booked for about 2 years........

However, I see smaller pots not shortening flowering time. I think below 1 gallon, there is a limit to the soonest it will flower. I too have suspected larger pots will flower later when the root mass hits critical size. This test sounds interesting. A lot of possible variables though also. Strains may vary. Would be great, though likely not feasible, if we all grew the same strain.

The same strain would be preferred. It would not likely matter if each replication was in a separate environment though, provided each set was in the same environment to its self. We would still get usable data about root:shoot and timing. But it's a big endeavor for home growers to be sure. The vision I had was to use this as preliminary data to predict exactly whether it was something like roots curling up (similar to gravitropism) or root ratio. Once we absolutely nail it down to an organ or mechanism, we can repeat it, and then have enough information to do the really hard tests like DNA marker identification, or at least physiological system identification.

currently we all have landed on the same notion though, root mass and flower shift are related. It could be hormonal based on root physiology, it could be something like it is for photo plants. In photos the daylight clock is in the leaves and florigen is created there, and translocates up the phloem to the meristem where it induces the flower morphology..

 

[Eclectic Elle]

Ummmmmm....
Cloning can net let’s say 10 decent clones of a branch in a quick turnover with high probability of genetic duplication... while tissue culture cultivation can give supposedly exact DNA replication with a hell of a lot more time and work... a leaf could net 50 plus cultures
Lmao
Mind you better starting culture means quicker finish and such
... multiple stages with varying lengths, where a clone is like hey there’s roots transplant it

Then there’s contagions and stuff which can possibly alter shit and stuff things up

Tissue culture involves growing some cells (callose) on a petri dish and then inducing roots and or shoots from it with hormones. I have done it once, but it's been a minute. It all has to be quite sterile too.

Great info, and enough detail to make me want to go remind myself of what I learned in biology (well overdue). Cheers guys!

 

[FullDuplex]

We went from a root test to tissue culture...seems that things took off in here rather quickly. I need to do more reading but i at least thought that i would drop in for an update.

Girls are healthy and looking good. Little sis is cathing big sis. She still has some height on her but the growth rate is amazing.

 

[trailanimal]

Time to show her off a bit so the fans are pretty good size the phone for reference is iPhone 6

@Waira here are a few more shots from tonight. Got her during leaf lift so you can see just how pretty she is.

Overhead

oh yeah!