This is an avenue I plan to explore in the future. If you are interested in that route, I highly recommend finding a DIYBio community to join. I plan to when timing permits.
Genetically manipulated Cannabis
- Thread starter Daniel king
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This is an avenue I plan to explore in the future. If you are interested in that route, I highly recommend finding a DIYBio community to join. I plan to when timing permits.
colchicine treatment attracts me because of its very simple yes or no way of working. if I treat a seed with it, either it will die or it will be polyploidal. if I can prove to myself that polyploidy plants are inherantly superior to diploids, then id never look back.
have you treated yourself yet to the awful film stoner express? if not you should. its ofcourse a joke, but a giant seed grows into a giant plant overnight. this it what im picturing with polyploidal autoflowers. not to any literal extent, but the movie would give you a laughable idea of what I mean
have you treated yourself yet to the awful film stoner express? if not you should. its ofcourse a joke, but a giant seed grows into a giant plant overnight. this it what im picturing with polyploidal autoflowers. not to any literal extent, but the movie would give you a laughable idea of what I mean
and if you find a diy bio community that dabbles with bio engineering cannabis, let me know and ill join with you lol
I'm going to dabble with cannabis, the DIYBio community doesn't need to know. In the name of canna haha
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haha my sentiments exactly.
@Daniel king I just found this site called the odin and its a diy bio kit site, not exactly cheap but at least can get an idea of what's needed to start in the hobby.
lol I had not seen it, but I have now. That was a monster plant haha.
It reminded me of an idea I used to have..I used to wonder if I could graft or cross breed cannabis with hops, I think they're in the same family, in fact I just searched for and found great info here: https://www.autoflower.org/threads/crossbreeding-cannabis-humulus-extended-edition.211/
Anyway great thread got me really thinking.
CRISPR is a way to edit genes, to either 'numb' them, or letting them 'over-express', so to speak.
This sounds pretty difficult, but when you know a certain gene has the same code in every plant (and with plants having a common ancester, that is pretty likely, especially because you'll only need to know something close to the first (FW), and last (RW) 16 CTAG-code combination), it would be easy to check for without doing a full sequence. You could just order some primers, PCR the genes to see if they're there (which can be done by hand if you have the time and some precise heated baths) and then do a cheap elektrophoresis (costing around 150 bucks for a set, and as little as 10 bucks per run). If the genes are present, you'd see it on the gel, ending up at the expected location compared to a known 'ladder'/control.
If you can check if the genes you'd like are present like that, there's no need for sequencing. If the gene is present, and approximately the expected size, you can copy its code from a gene-database and just order a CRISPR set with primers. This means you'll have to do some reading on genetic techniques. There might be some DIY biohacking websites that give more information, but the basis of this knowledge needs to be solid in ones mind. A simple screw up could cost you the entire project.
This technique however, is something very, very illegal if you don't have the right paperwork. I've never seen anyone fined for it, because usually the people who have this knowledge, take some form of an oath. "Genetic modification has to have no negative consequences for the world, it's inhabitants and flora and fauna, and it has to contribute in a positive way to the world."
But then again, why on earth would you do this the hard way? Why engineer a slow growing plant, why not go beyond that?!
Build a freaking DIY Tungsten nanoparticles gene gun, extract trichome RNA/dna, PCR it, and shoot it into a yeast culture? Homebrewing is hip and happening, nobody will suspect a thing! Extraction kits are readily available and I guess the genes should be known by now.
As for the hops x cannabis hybrids: protoplast fusion seems to be the way to go, but that requires radiation. Either that, or embryonic rescue if there's a possibility on actual natural fertilization.
This sounds pretty difficult, but when you know a certain gene has the same code in every plant (and with plants having a common ancester, that is pretty likely, especially because you'll only need to know something close to the first (FW), and last (RW) 16 CTAG-code combination), it would be easy to check for without doing a full sequence. You could just order some primers, PCR the genes to see if they're there (which can be done by hand if you have the time and some precise heated baths) and then do a cheap elektrophoresis (costing around 150 bucks for a set, and as little as 10 bucks per run). If the genes are present, you'd see it on the gel, ending up at the expected location compared to a known 'ladder'/control.
If you can check if the genes you'd like are present like that, there's no need for sequencing. If the gene is present, and approximately the expected size, you can copy its code from a gene-database and just order a CRISPR set with primers. This means you'll have to do some reading on genetic techniques. There might be some DIY biohacking websites that give more information, but the basis of this knowledge needs to be solid in ones mind. A simple screw up could cost you the entire project.
This technique however, is something very, very illegal if you don't have the right paperwork. I've never seen anyone fined for it, because usually the people who have this knowledge, take some form of an oath. "Genetic modification has to have no negative consequences for the world, it's inhabitants and flora and fauna, and it has to contribute in a positive way to the world."
But then again, why on earth would you do this the hard way? Why engineer a slow growing plant, why not go beyond that?!
Build a freaking DIY Tungsten nanoparticles gene gun, extract trichome RNA/dna, PCR it, and shoot it into a yeast culture? Homebrewing is hip and happening, nobody will suspect a thing! Extraction kits are readily available and I guess the genes should be known by now.
As for the hops x cannabis hybrids: protoplast fusion seems to be the way to go, but that requires radiation. Either that, or embryonic rescue if there's a possibility on actual natural fertilization.
All the reading I've done comes to the conclusion that its a waste of time. The odds of a plant thats even worthy of growing are slim to none. If these types of mutations were beneficial, nature would have done it already.
I think there are better avenues of research in plant modification.
I think there are better avenues of research in plant modification.
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