Cutting Edge Cloning: Tissue Culture Propagation

[Powerful14]

Tissue Culture Cloning. Seems no one gets it. This may be a game changer if it can work with autos, and I have a few ideas if it doesn't work the first try. I even tried to spark interest in it for the AFN Test Team to get product AFN test it. The reaction its was ohh cool...... HELLO! THIS may be ( I'm not saying it is but... ) a game changer in growing autos. Its not hard to do. Keep everything sterile and all should be good.

But I'm going to jump in to this by starting with a photo period, a very special one. Ducksfoot. VERY hard to get in the USA, or anywhere else for that matter. After having sample the bud, and seen this beautiful plant's growth patterns, I have to use it! I've sIt will take 2-4 weeks to see results.
I'll take more pics and post later.

Does anyone realize what this could mean to the community if we can clone autos this way?? Stay Tuned!

Very interested I love cloning plants have not for a couple years but this was my first venture with pot plants.. would be huge for everyone if we could find a way to clone autos!!

 

[pop22]

Things have finally calmed down here, Going to start a new batch this week! Had too many projects before to restart this, but I'm going to keep this going till I succeed.

 

[Hillbilly grower]

Wow keep us posted,clone autos would be a great discovery. Good luck and I look forward to your progress. As always Peace from hillbilly grower [emoji1308][emoji1303]


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[pop22]

I've not had the time to get back to this just yet, I will after I open my greenhouse and then have room inside to work. I have the supplies ready! Just too much to do and too little space to do it in.

 

[Eyes on Fire]

LOL dont I know it lol sounds like my place lmfao

 

[KapScot]

I thought about this a while ago, and have had experience in micro propagation and cloning (now don't all laugh at once but it was potatoes not weed).

But the principles and objectives remain the same.

TO create a cloning stock...

So back to our tatties - well we had hundreds of varieties and they were all 'microplants' (think BONSAI ON STEROIDS) growing several-up in small sample tubes *the kind your doctor wants you to pee in (never understood how girls did... nvm).

These plants were stored in racks under early T3? tubes (the finger skinny ones) in incubators. Except our incubators were set to 4-5 degC. In this environment and with some padding in an alar nutrient gel (in the form of Manitol if I remember - a complex sugar that helps slow Osmosis and also helps protect cells from low temps) we could actually produce tiny little potato plants... You could either take stem cuttings from the tiny little leaves (every nodes a cutting) or they even produced little tubers that you could scalpel apart and create new plants from each 'eye' of the potato microtuber. Once taken out into a normal growroom and removed from the Manitol plants would revert to normal type.

Now I'd love to try an Auto in that environment << Would it just go full-term flower in microscale???
The Jam Jar Grow off ha ha << whole plant must be fully mature and contained withing your jar with the lid closed.

Alas all this weed growing stuff is frowned apon here - so no Grant fo that research lol...

Pretty sure it would be OK for Photo's but what the Ruderalis biological clock of the Auto's would do - is why we have threads like this.

 

[MaineGrower]

Tissue Culture Cloning. Seems no one gets it. This may be a game changer if it can work with autos, and I have a few ideas if it doesn't work the first try. I even tried to spark interest in it for the AFN Test Team to get product AFN test it. The reaction its was ohh cool...... HELLO! THIS may be ( I'm not saying it is but... ) a game changer in growing autos. Its not hard to do. Keep everything sterile and all should be good.

But I'm going to jump in to this by starting with a photo period, a very special one. Ducksfoot. VERY hard to get in the USA, or anywhere else for that matter. After having sample the bud, and seen this beautiful plant's growth patterns, I have to use it! I've sIt will take 2-4 weeks to see results.
I'll take more pics and post later.

Does anyone realize what this could mean to the community if we can clone autos this way?? Stay Tuned!

I'm mixing up tissue culture solution and looking at specifically making a medium that is made for callus mass creation. That way you should be resetting the material into an undifferentiated mass of cells, that an then become roots/shoots, which I would assume resets the biological clock of the plant.

 

[pop22]

I wish I could find time to get back to this the last few months have been too much chaos to do this. I'd love to see you post about this!

I'm mixing up tissue culture solution and looking at specifically making a medium that is made for callus mass creation. That way you should be resetting the material into an undifferentiated mass of cells, that an then become roots/shoots, which I would assume resets the biological clock of the plant.

 

[HemiSync]

I'm mixing up tissue culture solution and looking at specifically making a medium that is made for callus mass creation. That way you should be resetting the material into an undifferentiated mass of cells, that an then become roots/shoots, which I would assume resets the biological clock of the plant.

Please keep us informed on how things work out. Like @pop22 I too would like to get more knowledgeable on this subject, but have not had the time for it.

 

[CTb1]

I've done tissue culture before ... Not 100% sure it will work on autos... But I think it will considering you are capturing the plants DNA

Here's some legit information so everyone can give it a shot.

Chemicals like colchicine or sulfuran can induce chromosome doubling leading to polypoid cells which have the potential to be bigger and stonger than their parents.

You could then use silver thiosulfate to create feminized seeds from the plants and go on a poly-pheno hunt! Or do that on any plant.

I was just thinking of trying this
@HemiSync @pop22 @iampepe @SpliffScot @MaineGrower @Powerful14