Bob's Journey into Hybridisation and Genetics - For Dummies

[Bob's Auto's]

Small update for ya guys...

While shooting the pictures for the update above, I noticed quite some gnats flying around. Since this isn't flying doctors I mixed up some water with Spinosad. I use Conserve Garden from Edialux, as this is the easiest for me to get my hand on. And you don't need much of the product. 0.8ml/L water to be exact. Each pot got 100ml poured directly over the soil. Do this when the soil is already wet, so the product get's taken up by the soil and doesn't run down the side of the pot.
After applying the Spinosad to the soil I decided it was a good idea to spray the ladies one last time with the Boom Boom Spray. I used 1ml/200ml water mixed in a sprayer...More then enough to get every plant soaking wet from top to bottom. Top-tip, First start spraying the underside of the leaves and then continue with the topside.

Even caught myself in the act

Afterwards, I set the extraction fan at 50% and turned on the UV light to keep the temperatures up in the 24°C / 75°F range. This took about an hour and half and now everything is running like before, with that one exception that the extraction fan is turned up by one percent. The act of spraying the plants with the Boom Boom Spray is almost immediately noticeable. The plants get a boost of energy and I hope by doing this the boost will be in the form of more and/or thicker buds...
Anywho...This is the last time I'm using the spray in this grow as at this stage in the grow, there is always a risk for getting mold inside the buds. That's why I wasn't afraid to use my UV light for extra heat. But also for it's relative harmful light spectrum. Everything went from soaking wet to normal in the timespan of 90 minutes. So...I think I'm fine


Regards,

Bob

 

[Bob's Auto's]

Hey Guys,

Everything is going it's steady pace. The main cola's are starting to expand wherever the pistils have started to subside...On the lower ends of the plants pistil production is still going steady, so we're not at the finish line at all. But it sure as hell is looking very scrumptious

One bit of bad news is that my time lapse camera has given up and is gone to greener pastures...I've replaced it with the new one I bought, but this means I'll have to resort to switching SD cards every time I go to the tent...The annoying thing is, to get to the SD card I need to unscrew the camera from the tripod...So in short, another step back...

These ladies are piling up the trichomes though...

...Healthy looking and enjoying the new 30 Watt UV light, which runs two hours a day, once at 8am and once at 8pm. This has been running like this for a while now and I'm wondering if I should raise the amount or not? ie, might run it four times a day for one hour...What's your thought on this guys?

The ladies are still drinking 2.5L/0.66gallon per day divided between the nine of them...To the 5L bottle of dechlorinated water I add 15ml Bio PK 5-8 and 25ml Orgatrex. Shake well before watering, because when left for 24 hours there's quite a lot of sludge which collects at the bottom of the bottle. All pure stinky goodness fattening my buds

Even the fanleaves are turning crusty with trichomes...

Yummie

I can look at this picture for hours...This is one of the lowers of my seed mothers...

Anyway, no time lapse today, but hopefully I'll be back with them very soon...It's just going to be so much more work extracting the pictures from the damn thing...pfff....
Ya'll have a fine evening/day/morning and I'll see you next time.
Oh, before I forget... @Antonio_DutchPassion, you have fun at the Cannafest in Prague my man!!


Regards,

Bob

 

[Bob's Auto's]

Hey guys

Everything is going smoothly inside the tent, but that's old news right?
Every day I divide 2.5L water, with Orgatrex and Bio PK 5-8 added to it, between the nine ladies inside the tent.

I've also added another one hour shift for the UV light. Now the lights come on every eight hours for one hour at a time. I feel the ladies are responding very well to this treatment. And I've not seen any issues arising from this treatment.

Also cleaned up the lowest branches from each plant. The budsite weren't going to amount to anything and the energy is better spent somewhere else.

Yup, I'm satisfied with this cross. Just have to be patient for a couple of more weeks until I can chop them down.

In the meantime I've started to collect White Widow from several other sources as well. When reading about the history I found out that the Fem seeds from Greenhouse aren't probably the real deal. Since Franco past away (RIP), the company never seemed to have released any new cultivars and is focussing it's buisiness to feminizing their stock. Reading further about the history, the chances that Mr.Nice Seeds, The Sjaman and Dutch Passion are the only ones in posession of the real parent's, or at least clones from the original stock is higher then the other way around...So I'm laying my eggs in three baskets. At least I hope so*. Three versions... A blind test, unbiased...I will set something up where I can't/won't know which is which.
In the end I'm searching for that one I like and that's a personal hunt...using scent, vision and touch to select what I finally want on my plate.

*Tonight I came upon an auction for a White Widow reg at Mr.Nice Seeds which I'm hoping for I will win.
With a bit of luck I can get my hands on that 15-pack of seeds, together with the reg seeds from Ingemar (The Sjaman) and the Dutch Passion Regular seeds, which Antonio is fixing me up, God bless his heart , I must be able to get myself some nice pheno's to hunt through, even though I don't have a lot of space inside my tent, I'm still gonna go for gold.

The idea is to start the seeds around November 15th in solocups filled with sterile seedling mix. So when today's ladies are harvested I can immediately fill up the tent with a new batch of youngling's.

This will be my first Photo run you guys, so I'm already begging those with the knowledge to help me out as this kind of growing will probably give me all kinds of headaches in the beginning... ie, I've never grown with lights out, so don't know what to do and what to expect VPD (temp/humid) wise...

Ok, now back to this grow...Lights out for those extra special pictures...Kinda burlesque no?

This is what we are after guys...Nice plants, that flower quickly and are full of trichomes...And still a couple of weeks to go...I would guess...Three weeks...

Ok, one more before I show you the time lapse.

Bear in mind that this new camera only takes pictures every one minute against the old one, which could wait 5 minutes before shooting the next... So I've upped the speed to 120fps and the duration = 53 hours. On the plus side...The resolution went from 4000x3000 to 5120x3840 pixels, which should help with the resolution and quality of the images.
So without further ado here you go guys, enjoy
Sorry guys, no time lapse today...After four hours of compiling I got a clip which hangs...Not for today anymore. Gonna rest a bit and set an alarm.
Already won a Dreamtime 15-pack for 17€, so wish me luck with White Widow






Regards,

Bob

 

[Bob's Auto's]

Hey Friends,

Another day another quick update.
Some girls are starting to look the look...

...While others are still pushing out pistils...

I'm guestimating that these faster ladies still need two to three weeks...Looking at the trichomes...

...The others...A week or two longer...

Regards,

Bob

 

[Bob's Auto's]

Hey Guys

Figured out what was wrong with the old camera. The exploded battery broke the sd card and the outside cover, but after replacing the card and battery it booted right up... So without further ado...Speed = 30fps, duration = 56 hours. Enjoy

Regards,

Bob

 

[Bob's Auto's]

Hey Guys

Not much to to say about this grow as everything is slowly coming to an end...I've stopped giving nutrients for a while as I feel there's still more then enough in the soil. We'll see in a couple of waterings if I will be adding some Bio PK 5-8

Oh, I also upped the amount of UV light to four times a day, each time the light runs for one hour. I feel the plants do respond more heavily on this lightspectrum by producing more trichomes, but then again, I'm biased...

Ok, not the parts you guys are interested in...

One of the slowest girls...To each there own

And the very slowest of them all...

To sauce things up here's another time lapse. Speed = 60fps, duration = 67 hours.
As you can see, the plants are concerned with only one thing...Epansion

Regards,

Bob

 

[Bob's Auto's]

First off, thanks go out to @Lil Dab for the rep points.
I harvested my first plant today...She looked ready enough and I couldn't wait any longer...

Her trichomes looked mostly amber, but I only got one reasonable picture of it...

The rest of the girls still looked like they could have a couple more days...

The seed mother...

And the other seed mother...She began as my backup as I prefered the smaller pheno, but finally...this one will have a far more larger yield.

Let's end today's post with another...yup, you've guessed it, time lapse...Speed = 60fps, duration = 100 hours. Enjoy

Regards,

Bob

 

[Bob's Auto's]

Hey guys,

After everyone went to bed I sowed the seeds for this next project.
This project is inspired by Mephisto's Port & Stilton, which I had the honor of growing one time and that was quite a smoke. Since Mephisto isn't gonna make these seeds anytime soon, I feel obliged to give it a go and try to recreate what I enjoyed so much.
Port & Stilton was a hybrid created from crossing a Double Grape x Stilton Special. I can't get any of those, but I do have several Double Grape crosses in my library.
From all the cultivars only the (Double Grape x Toof Decay) x Sooty Bride is a regular and from these seeds the male genome donor for all the other girls will be selected. Like always, I'll be selecting on several basis and this starts at germination.
This is what I sowed and what I want to end up with. For those of you that know my setup, this amounts to 16 plants. Which fit perfectly inside the 3.5L pots.

Double Grape x 3 Bears OG	4 > 3
Slaughtered Lam	10 > 3
Skywalker OG x UK Cheese	11 > 3
Cheese XXL S2	5 > 3
(Double Grape x Toof Decay) x Sooty Bride	11 > 3 + 1

After germination the sprouts will be sowed into a germination tray with humidity dome and built-in lights...so the first selection will be to see which seeds emerge first and how perty they look. The remaining sprouts/unhatched seeds will go to the chickens.

Regards,

Bob

 

[Bob's Auto's]

I'm pollen-chucking with some polyhybrid regulars (Northern Cheese Haze x Chem Candy). Then I'm going to do three open pollinations backcrossing males from each generation with a fresh group of sprouts from the DG seeds I made and see what happens.

Sounds interesting. Creating polyhybrids opens up the entire spectrum of genealogy in the F1 & F2 generations. Backcrossing to an original parent will bring that parent's genes out more. Just smoking the first quick dried buds from the plant I harvested yesterday, which is a Bx to Mephisto's Wedding and boy-o-boy...Even badly dried this is already amazing bud!! wooopwooop.

So, while down there, watering the ladies I thought to myself, why not take a couple of pictures for my AFN friends...
First couple with the lights out, these girls look frosty huh?

They are so close you guys...Senecense is setting in looking at the different leaf colors.

And the amount of frost accumulating is just ridiculous!

In the left corner the larger pheno seed mother...Checked every crusty leaf for that tell tale sign of mold. Toptip you guys; lightly pull on that said leaf and if it comes loose after a small tugg, take a look at the stem. If it's grey, you've got mold. If the stem is a nice straight cut, you are good and that's just the plants way of getting rid of them leaves.

Nice clayx-to-leaf ratio. And if I ca, find some cheap ass trim tray I feel there's loads of trichomes to be harvested from this cultivar. My grinder has a sepperation screen and only after one bud with a load of dried leaf it left a pile of trichomes good enough for vape test...

Even the runt of the litter is looking mighty fine here...

Just before watering the lot. I find it easier to remove two pots so I have free reign with my watering hoze. Yup, each plant is hand watered from start to finish. This gives me the opportunity to check each plant from top to bottom while doing so.

A couple of bud shots for you pr0n lovers...

This large pheno seed mother is looking very scrumptious, if I may say so...

Now It's just waiting patiently for the ripening of the trichomes

What do you guys think? Is this cultivar good enough to be crossed with the Widow of my dreams?

Regards,

Bob

 

[Bob's Auto's]

Oh-boy-oh-boy-oh-boy-oh-boy....We're getting close my friends!!
Many of the leaves are showing senescence and are turning colors...Pistils are shriveling up and turning color. Beautiful times ahead boys and girls.

This is the first seed mother...I'll have to keep her a while longer until the seeds are ripe or until she gives up...

Trichome wise she still can have a couple more days...

Second seed mother... Looking so yummy, oh my gosh!!!

The same for this one...If I had to choose based on the trichomes, this one would also come down in a day or two...

This one is as ready as she's gonna be...

A nice mix of milky and amber. And although only one picture, the whole plant is looking this way.

Another one thats completely ready...

There's always an exception and in this case it's this lady. She's not quite ready yet so I'll keep her as long as I'm keeping the seed mothers.

Ok, probably the last time lapse for this project so enjoy!! Speed = 120fps, duration = 64 hours.

Regards,

Bob