Photography CBD 1:1

[Twenty20 Adam]

You are saying you stabilized a 1:1 CBD:THC as a true breeding trait in a line? That would be pretty rare and interesting. It would have to be a recombination at the linked THCAS/CBDAS gene region...

Talking about stabilizing the 1:1 ratio. The first few generations are wild. We get everything from 20:1 to 1:20. After choosing 1:1 (approximate) parents and breeding them together for multiple generations the outliers begin to disappear. The ratios start to range from 1:3 to 3:1

Yes, need to know this as a good friend as he was diagnosed as stage 4 bone cancer... Not terminal as of yet. Will need to grow some out for him!

Yes. Seeds will be ready shortly. Stay tuned and Ill send you some for you/your friend ASAP.

Congrats on the results after that many years of work, it must feel great
Will there be seeds for sale at some stage?

HI,View attachment 1262340
Yes they will be and we are also kicking off the year with a big "free the seeds" campaign. I'd love to get the AFN community involved in any way possible. It's totally free, no strings attached. We do a big free seed campaign every year but this year it is with feminized autos.
Here is a pic of a different pheno

 

[JSH1973]

Talking about stabilizing the 1:1 ratio. The first few generations are wild. We get everything from 20:1 to 1:20. After choosing 1:1 (approximate) parents and breeding them together for multiple generations the outliers begin to disappear. The ratios start to range from 1:3 to 3:1

Yes. Seeds will be ready shortly. Stay tuned and Ill send you some for you/your friend ASAP.

HI,View attachment 1262340
Yes they will be and we are also kicking off the year with a big "free the seeds" campaign. I'd love to get the AFN community involved in any way possible. It's totally free, no strings attached. We do a big free seed campaign every year but this year it is with feminized autos.
Here is a pic of a different pheno

In my next grow starting in January I'm planning to include one 1:1 CBD/THC and one CBD only, if you do come to an arrangement with the forum mods/owners about members trying your strain, then I would love to be considered

 

[GrowingHigher]

Talking about stabilizing the 1:1 ratio. The first few generations are wild. We get everything from 20:1 to 1:20. After choosing 1:1 (approximate) parents and breeding them together for multiple generations the outliers begin to disappear. The ratios start to range from 1:3 to 3:1

That seems pretty strange to me. Generally, high THC plants have a non-functional or poorly functional CBDAS, and most high CBD plants have a functional CBDAS and THCAS (some have nonfunctional THCAS as well), with minor THC production in both from CBCAS genes. So, maybe you are selecting for a less functional or leaky CBDAS, which could have been in the Remedy parent if it was heterozygous for a highly- and partially-functional CBDAS. Otherwise there would have to be de novo THCAS/CBDAS alleles (or of some gene affecting thier regulation) arising via mutation during the breeding process which has shifted efficiency of one or both CBDAS/THCAS enzyme variants. Unless something I have no understanding of yet is happening.

Was this a full-sib breeding scheme for each generation of selection?

 

[Twenty20 Adam]

That seems pretty strange to me. Generally, high THC plants have a non-functional or poorly functional CBDAS, and most high CBD plants have a functional CBDAS and THCAS (some have nonfunctional THCAS as well), with minor THC production in both from CBCAS genes. So, maybe you are selecting for a less functional or leaky CBDAS, which could have been in the Remedy parent if it was heterozygous for a highly- and partially-functional CBDAS. Otherwise there would have to be de novo THCAS/CBDAS alleles (or of some gene affecting thier regulation) arising via mutation during the breeding process which has shifted efficiency of one or both CBDAS/THCAS enzyme variants. Unless something I have no understanding of yet is happening.

Was this a full-sib breeding scheme for each generation of selection?

The Earlier generations were half-sib when we were using males but the later generations were full-sib after we started reversing females and choosing the parents selectively based on a number of factors but most importantly CBD:THC ratios.

 

[GrowingHigher]

The Earlier generations were half-sib when we were using males but the later generations were full-sib after we started reversing females and choosing the parents selectively based on a number of factors but most importantly CBD:THC ratios.

If you don't mind... how many total generations are we talking? Any idea what's happening? What kind of ratios for the 1:3-3:-1 progeny? Is it either-or, or are there any distinct groupings within that (like ~1:1s)?

Any plans to do any genetic testing on this? I would be very curious about what's happening here.

 

[Twenty20 Adam]

If you don't mind... how many total generations are we talking? Any idea what's happening? What kind of ratios for the 1:3-3:-1 progeny? Is it either-or, or are there any distinct groupings within that (like ~1:1s)?

Any plans to do any genetic testing on this? I would be very curious about what's happening here.

It's complicated. We started the project years ago with photoperiods. 2 years ago we started working the autoflowering trait into it. The results have certainly been a roller coaster and after reading your previous responses it makes more sense. originally the outliers were predominantly high thc (very low CBD). Now, we see very few of those where the outliers are more in the 1:6 ~(12% cbd 2% thc) range . To answer your previous question about half-sib, full-sib, we also had a few generations that were selfed. We are about to harvest a small sized seed production run and will be publishing the ratios for the customers so I will share with you then.

 

[Chili]

This looks tasty, I’m interested.