Pollination is immediate. So if your brushing on your pollen, by the time you brushed it on then you can spray... if your going airborne then sky is the limit... so your answer has variables... 1min for brush on, airborne when ever you think she has got it good...
It the same as sperm hitting the egg, once they meet game over, or should I say games begin... Once that pollen lands on the pistil your golden...