CS breeding

[TreyDogg]

 

[TreyDogg]

Alright. Cut he she down. Cut and saved nugs with male sign set them to dry to the side. Rest of plant in the trash.

Pulled out the bud after a couple days and gave it some shakes. Could see pollen come off and I think we have pollinated girls. Looks already like something has happened. Some of the whitehairs have turned red and its very early. I rubbed that bud all over the small hairballs hope that will help them all get some seed.

 

[mikey2017]

Alright. Cut he she down. Cut and saved nugs with male sign set them to dry to the side. Rest of plant in the trash.

Pulled out the bud after a couple days and gave it some shakes. Could see pollen come off and I think we have pollinated girls. Looks already like something has happened. Some of the whitehairs have turned red and its very early. I rubbed that bud all over the small hairballs hope that will help them all get some seed.

I've got one I turned from using 2 silver coins and a 9 volt battery and I'm wondering when I should collect pollen..

Sent from my H1611 using Tapatalk

 

[regen]

Hi there!

Sorry for bombing the thread (and my broken engrish) Just name of the topic pulled me here. I want to try CS breeding too. I cant order seeds anymore and my photo mothers are dead (sadly). I have some autos left and planted 2 of em. I think 1 x white widow auto and 1 x white widow x power plant auto afaik.

If i cs ww (partial) and use polens on other plant, would this be a x-breeding? If yes, do you recommend this? Or can i use polens on original plant, if yes, what about the time line? I mean since its an autoflower, things are a bit under sands of time. What if i let the plant do its thing and pollinate the whole flowering chamber? Are those pollinated plants still smokable? The tent is infected after that? Or too hard to clean up? Should i keep breedinig on new generations and are these seeds identical to their origin? Or any mutations are expected? (On same plant, or x-cross)

Edit: hmm, i have an idea;
I will lst those plants and i can take clones too. Yes,you can clone autoflowers, but not like a photo plant. The idea is, i take clones when she is in preflower and use those clones for cs breeding. Neither i lose my endproduct, nor any contamination danger. I took lots of clones when i grow autos and they did just fine as expected. I think when i type more ill find all answers i need lol.between, if you keep the autoflower under 24/0 it delays the flowering phase. At least that happened to my plants and i will use this as a benefit this time.



Again, sorry for my ignorant to bomb your thread, i am really helpless at this point. After these seeds there is no growing fun for me anymore. Hope you understand my concerns

Peace.

 

[fryge]

If i cs ww (partial) and use polens on other plant, would this be a x-breeding?

yes(unless the other plant is a clone of the reversed plant, then they're genetically identical and it's selfing). except for the sex chromosome, it works exactly the same as using a male plant.

If yes, do you recommend this?

depends on what your goal is.

Or can i use polens on original plant, if yes, what about the time line?

I don't really know about CS, I've only used STS. with STS you have to count on about 2 week delay for the reversed plant, since it will be ' frozen' for a while after you apply sts, and then after a while(1-2 weeks) it'll start growing again. the rest is similar to a regular male. if you want maximum amount of seeds, introduce the male/reversed female around week 3-4 of flower of the mother.
you can either take clones and reverse one to pollinate another to self, or treat part of the branches of one plant/use a low dose so there are only a few male flowers, so it's all on one plant. both work, I think using clones would give slightly better seeds(I noticed on partially reversed plants the seeds mature less and stay smaller, but that's mostly within the same buds, if you treat one branch and have another untreated branch it should work better, and they still mature enough to be viable seeds).

I mean since its an autoflower, things are a bit under sands of time. What if i let the plant do its thing and pollinate the whole flowering chamber?

yes you could do that, but you would have loads of seeds, probably way more than you need.

Are those pollinated plants still smokable?

yes, the same as any seeded plant. to be sure I wouldn't smoke the CS-treated plants, but plants pollinated with the reversed pollen that didn't get treated with CS themselves are fine. but like any seeded plant, it'll have lower yield and you have to remove seeds before smoking.

The tent is infected after that? Or too hard to clean up?

I can't say much about that, don't have an indoorspace, I just grow outdoors and sometimes in my windowsill(for pregrowing in spring or for seeds). but I wouldn't worry that much about it, I guess if you wipe all the walls and floors down with a cloth with some water with all-purpose cleaner irt should be fine, or spray everything down with some water.

Should i keep breedinig on new generations and are these seeds identical to their origin?

depends on what you want to achieve. but no, don't count on them being identical.
even if the mother and father are identical genetically, you'll still get differences in the offspring, as long as the parent isn't completely homozygous. so a heavily inbred line will have offspring from selfed seeds that is very similar to the parent, but if it's not already stabilized, you'll still see a lot of variation in the plants coming from selfed seeds.
but each generation of selfing(+selection for the plants you like most) you'll get more homozygosity, and so each generation becomes more stable if you keep selfing(in plant breeding there's a rule of thu,b you need about 6-8 generations of selfing to stabilize a line)

Or any mutations are expected? (On same plant, or x-cross)

this is a bit of a though question, I have 2 answers depending on how I interpret the question.
ignoring weed-terminology and going by definoitions from biology, I would say no: mutations occur constantly, but it's not really something you'll see a lot of, and the rate of mutation is constant, so you won't get more or less mutations due to how you make the seeds, unless you use mutagens like colchicine.

however, I think I know what you mean, and it's not that. the thing is, mutations happen constantly, but they usually stay invisible. when people talk about 'mutants' with weed, it's not like that mutation only just happened, it was there all along, it just didn't show.

and in that case, yes, it's likely you'll see more 'mutants' if you keep selfing seeds.

the thing is, usually mutations are not good. usually, something gets broken('loss of function' in biology terms). however, since there are 2 of every gene, usually that 2nd 'healthy/normal' gene is enough so you won't see anything wrong. and since there is nothing wrong, there is also no selection against the mutation, so it stays and gets passed on.

but by selfing you'll get more homozygotes, also for those mutated genes. and that's when you start seeing 'mutants'. but it's not new mutations, it's existing mutations showing, it's inbreeding depression.
however, you can't do stabilizing without inbreeding, so it's a trade-off. you can focus on preventing inbreeding depression at all costs, but then you'll never stabilize anything, or you can stabilize to the extreme but then you'll have a mutant inbred redneck-plant.

 

[The Elvis]

Alright. Cut he she down. Cut and saved nugs with male sign set them to dry to the side. Rest of plant in the trash.

Pulled out the bud after a couple days and gave it some shakes. Could see pollen come off and I think we have pollinated girls. Looks already like something has happened. Some of the whitehairs have turned red and its very early. I rubbed that bud all over the small hairballs hope that will help them all get some seed.

She looks amazing!!!! i would save those nanners with pollen in a dry glass jar just in case you wanna rub on another female in the future!!! Don't trash the pollen, that's fem pollen..
I want some.. I'm kidding but awesome thread!!!

 

[regen]

Wow thats the info i need. I feel released

Due to draconian rules here, where i live, it is hard to order seeds and i am out of my stash included seeds. I will definitly clone my girls and will use cs on em. Cloning autoflowers is easy. You just get one crafty flowering stem up to 2 feet with some side branches. So 4-5 clones will do the job and i will not lose any smokable material. Seems worth to try.

Thank you again

 

[regen]

Hi guys,

As promised, i took a clone from my auto and kept spraying with cs. The solution is about 44 ppm and i am still spraying daily 1-2 x time. I hope, it works:

 

[regen]

Hell yeah!!

Felt like i play diablo II. LoL. I just harvested 5 bananas and bam. Now i need to take care of thousends lol.

 

[regen]

Hi guys,
I germinated some of cs-seeds and they are quite happy and vigorous. I think oldest one is at day 15 or something. They are much more faster than their mums. One of them looks sativiish . Pics: