New Grower Cloning autoflowers from leaves w/BAP

[HomeGrownRx]

this is a topic i brought up a while back, im actually doing experiments now so i thought id start a nice fresh thread.

vie seen plenty of people root a leaves on other sites. however, i have never seen anyone produce a full plant from a rooted leaf, no stems ever form.

I hypothesize that the external application of 6-benzylaminopurine (bap) to rooted Cannabis leaf will result in the production of stems on said leaf.

this is done in plant tissue culture all of the time for this exact purpose. The difference between tissue culture techniques and this (if it works) is that it can be done at home in a non sterile environment with ease.

below are pictures of a photo period grand daddy purple leaf (cut before the node) with rooting hormone on it being placed in 1 media of 1:1 of perlite:coco coir. i will water it with aerated tap water.

if and when these leafs strike roots i will apply BAP by putting it in the water i water it with, foliar spray and a paste (bap mixed with aloe). i hope that eventually this will result in a new grand daddy purple.

i did the photo gdp because i was afraid to cut my auto for an experiment, shes beautiful. I gave in and cut off one lead then another to even her out. The leafs were taken from my Fast Bud#2 plant growing in 1:1 perlite coco cut at aprox 1 month from seed. they were super thick and healthy, this strain is very vigorous!

im going to try not and peak at it for 1 whole week (i give my plants too much attention).

so if this works on the auto leafs, we can produce whole plants from leafs. that means no significant stress on the mother plant, more overall clones and a way to keep a solid clone of a much loved strain.

tell me what you think, id love feedback and advice.

i should have made the photo shoot with the autoflower. fast bud #2 is sooo much healthier looking than the super hyped grand daddy purple - photo.

 

[whatsgoodie]

Straight to hempy, eh? You get all that stuff sorted out on your last grow w/ hempy?

 

[SmashedbyHashish]

I don't think its possible, but I'll be watch for the out ccome...happy growing.

 

[HomeGrownRx]

my current grow is my first hempy grow. my autos are absolutely looooving it. my gdp photo is loving it as well but is quite disfigured because ive used her for a few too many experiments in too short a time.... you should see some of my cacti.

a callus roots and then produces stems. what im not sure about is whether a rooted leaf will act as a plant callus in tissue culture. if thats the problem, equal amounts of bap and iaa will be added to the leaf in order to first form a callus.

i haven't researched this stuff in w little while so im a little rusty. time to do some reading.

---------- Post added 08-08-2011 at 11:55 PM ----------

first result off google, ill find better stuff later.

http://www.springerlink.com/content/glc32cl8t48t2jlq/

 

[HomeGrownRx]

im not sure it will take so many tries. ill be using very high concentrations off both iaa and bap with a higher concentration of bap. if it doesnt work with ease i have no interest in it because its of no use to the average autoflower grower.

i have done it with sterile agar media (ms) with african violets.

here is the first thread on a google search of rooting marijuana leaves. http://forum.grasscity.com/advanced-growing-techniques/348417-cloning-single-leaf.html

taken from above thread

 

[iLuvIndica]

i dont get yea if you root a leaf how are you gonna get nodes to produce buds LOL doesnt look like from that picture that you can get buds you might be able to keep that leaf happy and alive but how are nodes gonna form?

 

[SmashedbyHashish]

That's my queston iLuvIndica, I don't see it. I also don't see the benefit in starting a leaf when I can start tops & have them in the flower room in 10 days if I like. If you do get a complete plant out of a leaf plz give a detailed report. Best a luck...

 

[hitman_64]

I have my doubts but to try and answer a couple questions. The hormone bap would promote the stems and cell division if any would occur at the cuts on the leaf veins. It should be interesting and for my purposes I hope it works.

 

[whatsgoodie]

I wonder if the fan leaf were to root and eventually produce nodes, etc.. would it be the same as starting from seed? I.E: will it produce a full plant? I've gotten branches off of a Mi5 to take root and eventually form one big ass nugget, but the time it took vs how much bud I got wasn't worth it, although it was an interesting experiment.. a leaf itself, I wonder...

Also if it works, what would the difference be between a leaf from a flowering plant and a non flowering plant?

 

[HomeGrownRx]

my hope is that it will produce a full plant and that it will be of average dwarf size. i have no reason to think this however.

if it can be done from one single plant cell taken from a leaf using micropropigation techniques then why not this way? one way to find out.

the only real use it would have is if it did produce average or slightly below average sized plants, in which case it could be used for mass proliferation of identical plants. ill post updates, except them to come slowly though. a week is about as fast as most people repetitivley root clones. im hoping for within two weeks to have roots. fingers crossed.

next soon to strat project is florigen experiments. its so nice to finally have a place i can grow so i can do experiments. my plants grow next to my burbank potatoes and my luther burbank white blackberry plant. my family new the guy (one of many idols of mine) and he lived down the street. so i have to do some experiments in his honor even if they are underfunded.

im breeding round up ready black aztec corn right now as an experiment. i hope to make round up ready autoflowers. got to be one step ahead anyone wanting to kill our plants! feelings on this one?