Beneficial bacteria can survive in your media as long as it has a food source. I should add that when I re-inoculate every week or two I only do 1-2gallons and give each plant a cup full. I am moving towards Bennie teas now tho, I think that would be more cost effective. How is adding it to the hole when transplanting keep them alive better than when poured with water?
I'm no expert so feel free to correct thy thinking
IN my stoner reaserch it was shown to me that the bacteria has a short life span.
Thus one needs to keep applying bacteria to keep the bacteria level up.
Also note that bacteria need bacteria freindly medium and fungi do better in fungi friendly medium and those mediums are not the same.
Short stoner take
Bacteria are some what mobile as bacteria move centameters around to eat and shit
Bacteria are singe individual cell organisms.
Mykos spores grow together to form a interconnected organism that spreads throughout the soil
The bacteria shit
The Mykos grab the shit
IF the Mykos is in contact with roots the shit is passed to the roots and the root say
"Thanks for the shit, Mykos. Here's some sugar for you."
But this exchange only works if Mykos is in touch with roots.
The more roots touched the better it gets
So
Mykos needs root contact for it to be effective.
Just mixing in soil or watering into soil is not efficient.
one would be wasting their Mykos by not applying it in efficient way.
By placing it in a transplant hole the roots must grow through it and the Mykos grows and expands in the medium the Mykos ( fungi) feeds the roots as the roots feed the Mykos BUT the Mykos must be in contact with the roots.
Also, unlike bacteria
The Mykos needs a few weeks to grow
I didn't mean to imply that NONE of the mykos spores will get to the roots when watered in.
A much much lower percent will.
Of course the many mykos manufactures will say to water in cuz it wastes more mykos and then one needs to buy more mykos.
Personally I'd prefer to do what works best for me and the plants I grow
and I'm a cheap one.
And rather not just waste my expensive Mykos
But please do what you feel works best for you.
Pax
*******edit*****+ I am just using the word MYKOS (a brand name) cuz I can't spell mellayceium (sp??)