Autoflowering female pollinated by photoperiod male

[Arthur]

found this, gives an idea of mixing the first gen.

 

[Kneehigh85]

View attachment 831675 found this, gives an idea of mixing the first gen.

This helps show chromosome traits exactly how it’s written in my article I love how it’s very visual and puts it into perspective of how generations get to the results you’re looking for!


Sent from my iPhone using Tapatalk

 

[Tacoavenger17]

This may sound like sort of a daft question but,

Has anyone thought of using the home crispr in a box gene editing kits as a tool in breeding?

May be able to knockthe photo period expression right out in theory, unless I'm massive wrong lol

 

[fryge]

This may sound like sort of a daft question but,

Has anyone thought of using the home crispr in a box gene editing kits as a tool in breeding?

May be able to knockthe photo period expression right out in theory, unless I'm massive wrong lol

interesting idea, but I'm guessing no.
I've never heard of that kit, my first thought was it's probably a scam. I know how crispr works(not detailed, just roughly), and I once made a genetically modified E. Coli(not with crispr-cas9), and it seems pretty unlikely they managed to get a kit together to do that at home at a reasonable price.

I googled that kit, and it's actually pretty cool, seems like it's actually legit.

however, it's clearly meant as an educational thing, not to actually achieve anything with it. I've quickly read trough the instructions and list of what's included, and they include 'template DNA'. so you would have to replace that with other template DNA to change the target. I don't have enough experience/knowledge to tell you how easy/hard that's going to be, but I can make some guesses.
you would first need to know how the photo/auto trait actually works genetically. since it inherits as a simple recessive gene, it's likely it's determined by a single gene. but where in the flowering initiation chain is it? what is the mechanism?
a knock-out mutation is indeed likely, but it's probably a knock-out of some inhibitor, which would usually prevent flowering hormone from being produced(just a guess, don't take this as fact). but I would guess there's a pretty complex network involved with different feedbackloops, so you'll have to find the right step in that whole chain.
so then you need to know the sequence so you can add part of the sequence as target for the crispr-cas.
as far as I know there are a few sequences available of weed, but I have no idea how usefull they are. if you're lucky the arabidopsis-sequences can help you, flowering initiation by daylength is not sometthing unique to weed, and flower initiation is also something so fundamental it could be pretty conserved.

and if you can't find the auto/photo gene in the databases, you'll have to do more work finding it yourself.

however, I've read crispr-cas is more difficult with plants as with bacteria, since plants have many repeated sequences. crispr-cas works with a programmed target, but in that case there could be multiple other spots, in other genes, that look the same as the target.

 

[Fitzy]

So ive done the same with a few autos and in my experiance i had mixed autos in first gen i thought it was afluke so i tried a seperate photo auto cross just started building sites one next to it def a photo i plant a few per pot to cull the weak post a journal of them would love to watch i can show u the pics of mine if interested

 

[Fitzy]

Sorry that pick is auto to auto wasnt suppose to post that1